X-gal Staining on Adult Mouse Brain Sections

X-gal Staining on Adult Mouse Brain Sections.

Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique is regularly used, it requires high quality of specific antibodies and there is no good quality of antibody available for certain proteins. Alternativ...

Direct confocal acquisition of fluorescence from X-gal staining on thick tissue sections

X-gal staining is a common procedure used in the histochemical monitoring of gene expression by light microscopy. However, this procedure does not permit the direct confocal acquisition of images, thus preventing the identification of labelled cells on the depth (Z) axis of tissue sections and leading sometimes to erroneous conclusions in co-localization and gene expression studies. Here we rep...

X-gal staining of Drosophila embryos compatible with antibody staining or in situ hybridization.

the slow development. The low background especially applies for detection of digoxigenin-labeled RNA probes. When detecting identical probes labeled with fluorescein, the background is higher, presumably because of nonspecific binding of the fluorescein antibody (the higher background is detected with both BCIP and NBT/BCIP). In cases in which the investigated signals are of different intensity...

Non-nuclear staining of thick tissue sections.

Staining of toxoplasma in histological sections.

EXTRACELLULAR toxoplasma, although present in abundance in smears from animals infected experimentally, can only occasionally be found in sections from suspected toxoplasma lesions. In the eye, Wilder (1952a, b) has described organisms morphologically indistinguishable from toxoplasma in cases of granulomatous uveitis, but even in these sections the organisms were not numerous and were found on...